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KMID : 0364820180540030200
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Korean Journal of Microbiology
2018 Volume.54 No. 3 p.200 ~ p.207
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Studies of cold resistant glycine betaine effect on cold sensitive Bacillus subtilis mutant strains
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Kim Do-Hyung
Lee Sang-Soo
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Abstract
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At high salt concentration, glycine betaine is transported intoBacillus subtilis and growing rate of the cell is not suppressed.
Also according to recent studies, cell growth is maintainednormal growth rate at low temperature. Low temperature resultsin a stress response of Bacillus subtilis that is characterized bystrong repression of major metabolic activities such as translationmachinery and membrane transport. In this regards, genesshowing cold sensitive phenotype are cold-induced DEAD boxRNA helicases (ydbR, yqfR) and fatty acid desaturases (bkdR,des). Therefore to understand the effect of glycine betaine oncold growth of Bacillus subtilis, we investigated the effect ofglycine betaine on growth rate of these deletion mutants showingcold sensitive phenotype. Glycine betaine strongly stimulatedgrowth of wild type Bacillus subtilis JH642 and deletion mutantsof ydbR and yqfR at 20°C (190~686 min Td difference). On theother hands, glycine betaine does not show growth promotingeffects on deletion mutants of bkdR, and des at cold conditions.
Same cold protectant growth results were shown with theprecursor choline instead of glycine betaine. We investigatedthe effects of detergents on the cell membrane in bkdR and desdeficient strains associated with cell membrane. It was identifiedthat bkdR deficient strain shows retarded growth with detergentsuch as Triton X-100 or N-lauryl sarcosine compared with wildtype cell. Thus, it is possible that deletion mutation of bkdRmodifies membrane structure and effects on transport of glycinebetaine.
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KEYWORD
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Bacillus subtilis, choline, DEAD box RNA helicase, glycine betaine, Td
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